H2N2V1, Main, Exploration, bibRecord, 002970

Effect of temperature on the order of electrophoretic migration of influenza virus neuraminidase and nucleoprotein genes in acrylamide gels lacking denaturing agents.

Identifieur interne : 002970 ( Main/Exploration ); précédent : 002969; suivant : 002971

Effect of temperature on the order of electrophoretic migration of influenza virus neuraminidase and nucleoprotein genes in acrylamide gels lacking denaturing agents.

Auteurs : N J Cox ; A P Kendal

Source :

The Journal of general virology [ 0022-1317 ] ; 1978.

RBID : pubmed:690606

Descripteurs français

KwdFr :
- ARN viral (analyse), Gènes viraux, Protéines virales (génétique), Recombinaison génétique, Sialidase (génétique), Température, Virus de la grippe A (analyse), Virus de la grippe A (génétique), Électrophorèse sur gel de polyacrylamide ().
MESH :
- analyse : ARN viral, Virus de la grippe A.
- génétique : Protéines virales, Sialidase, Virus de la grippe A.
- Gènes viraux, Recombinaison génétique, Température, Électrophorèse sur gel de polyacrylamide.

English descriptors

KwdEn :
- Electrophoresis, Polyacrylamide Gel (methods), Genes, Viral, Influenza A virus (analysis), Influenza A virus (genetics), Neuraminidase (genetics), RNA, Viral (analysis), Recombination, Genetic, Temperature, Viral Proteins (genetics).
MESH :
- chemical , analysis : RNA, Viral.
- chemical , genetics : Neuraminidase, Viral Proteins.
- analysis : Influenza A virus.
- genetics : Influenza A virus.
- methods : Electrophoresis, Polyacrylamide Gel.
- Genes, Viral, Recombination, Genetic, Temperature.

Abstract

When subjected to electrophoresis at 33 degrees C in 3% polyacrylamide gels with no urea added, the nucleoprotein and neuraminidase genes of an H2N2 and H3N2 virus migrate as RNA bands 5 and 6 respectively. If the temperature of electrophoresis is increased to 46 degrees C, however, this order of migration is reversed.

DOI: 10.1099/0022-1317-40-1-229
PubMed: 690606

Affiliations:

Le document en format XML

<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Effect of temperature on the order of electrophoretic migration of influenza virus neuraminidase and nucleoprotein genes in acrylamide gels lacking denaturing agents.</title>
<author><name sortKey="Cox, N J" sort="Cox, N J" uniqKey="Cox N" first="N J" last="Cox">N J Cox</name>
</author>
<author><name sortKey="Kendal, A P" sort="Kendal, A P" uniqKey="Kendal A" first="A P" last="Kendal">A P Kendal</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PubMed</idno>
<date when="1978">1978</date>
<idno type="RBID">pubmed:690606</idno>
<idno type="pmid">690606</idno>
<idno type="doi">10.1099/0022-1317-40-1-229</idno>
<idno type="wicri:Area/PubMed/Corpus">000617</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000617</idno>
<idno type="wicri:Area/PubMed/Curation">000617</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000617</idno>
<idno type="wicri:Area/PubMed/Checkpoint">000539</idno>
<idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">000539</idno>
<idno type="wicri:Area/Ncbi/Merge">001230</idno>
<idno type="wicri:Area/Ncbi/Curation">001230</idno>
<idno type="wicri:Area/Ncbi/Checkpoint">001230</idno>
<idno type="wicri:doubleKey">0022-1317:1978:Cox N:effect:of:temperature</idno>
<idno type="wicri:Area/Main/Merge">002B25</idno>
<idno type="wicri:Area/Main/Curation">002970</idno>
<idno type="wicri:Area/Main/Exploration">002970</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en">Effect of temperature on the order of electrophoretic migration of influenza virus neuraminidase and nucleoprotein genes in acrylamide gels lacking denaturing agents.</title>
<author><name sortKey="Cox, N J" sort="Cox, N J" uniqKey="Cox N" first="N J" last="Cox">N J Cox</name>
</author>
<author><name sortKey="Kendal, A P" sort="Kendal, A P" uniqKey="Kendal A" first="A P" last="Kendal">A P Kendal</name>
</author>
</analytic>
<series><title level="j">The Journal of general virology</title>
<idno type="ISSN">0022-1317</idno>
<imprint><date when="1978" type="published">1978</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Electrophoresis, Polyacrylamide Gel (methods)</term>
<term>Genes, Viral</term>
<term>Influenza A virus (analysis)</term>
<term>Influenza A virus (genetics)</term>
<term>Neuraminidase (genetics)</term>
<term>RNA, Viral (analysis)</term>
<term>Recombination, Genetic</term>
<term>Temperature</term>
<term>Viral Proteins (genetics)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>ARN viral (analyse)</term>
<term>Gènes viraux</term>
<term>Protéines virales (génétique)</term>
<term>Recombinaison génétique</term>
<term>Sialidase (génétique)</term>
<term>Température</term>
<term>Virus de la grippe A (analyse)</term>
<term>Virus de la grippe A (génétique)</term>
<term>Électrophorèse sur gel de polyacrylamide ()</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>RNA, Viral</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Neuraminidase</term>
<term>Viral Proteins</term>
</keywords>
<keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>ARN viral</term>
<term>Virus de la grippe A</term>
</keywords>
<keywords scheme="MESH" qualifier="analysis" xml:lang="en"><term>Influenza A virus</term>
</keywords>
<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Influenza A virus</term>
</keywords>
<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Protéines virales</term>
<term>Sialidase</term>
<term>Virus de la grippe A</term>
</keywords>
<keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Electrophoresis, Polyacrylamide Gel</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Genes, Viral</term>
<term>Recombination, Genetic</term>
<term>Temperature</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Gènes viraux</term>
<term>Recombinaison génétique</term>
<term>Température</term>
<term>Électrophorèse sur gel de polyacrylamide</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">When subjected to electrophoresis at 33 degrees C in 3% polyacrylamide gels with no urea added, the nucleoprotein and neuraminidase genes of an H2N2 and H3N2 virus migrate as RNA bands 5 and 6 respectively. If the temperature of electrophoresis is increased to 46 degrees C, however, this order of migration is reversed.</div>
</front>
</TEI>
<affiliations><list></list>
<tree><noCountry><name sortKey="Cox, N J" sort="Cox, N J" uniqKey="Cox N" first="N J" last="Cox">N J Cox</name>
<name sortKey="Kendal, A P" sort="Kendal, A P" uniqKey="Kendal A" first="A P" last="Kendal">A P Kendal</name>
</noCountry>
</tree>
</affiliations>
</record>

Pour manipuler ce document sous Unix (Dilib)

EXPLOR_STEP=$WICRI_ROOT/Sante/explor/H2N2V1/Data/Main/Exploration

HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 002970 | SxmlIndent | more

HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 002970 | SxmlIndent | more

Pour mettre un lien sur cette page dans le réseau Wicri

{{Explor lien
   |wiki=    Sante
   |area=    H2N2V1
   |flux=    Main
   |étape=   Exploration
   |type=    RBID
   |clé=     pubmed:690606
   |texte=   Effect of temperature on the order of electrophoretic migration of influenza virus neuraminidase and nucleoprotein genes in acrylamide gels lacking denaturing agents.
}}

Pour générer des pages wiki

HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i   -Sk "pubmed:690606" \
       | HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd   \
       | NlmPubMed2Wicri -a H2N2V1

This area was generated with Dilib version V0.6.33.
Data generation: Tue Apr 14 19:59:40 2020. Site generation: Thu Mar 25 15:38:26 2021

Serveur d'exploration H2N2

Effect of temperature on the order of electrophoretic migration of influenza virus neuraminidase and nucleoprotein genes in acrylamide gels lacking denaturing agents.

Effect of temperature on the order of electrophoretic migration of influenza virus neuraminidase and nucleoprotein genes in acrylamide gels lacking denaturing agents.

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki

	Serveur d'exploration H2N2
	Attention, ce site est en cours de développement ! Attention, site généré par des moyens informatiques à partir de corpus bruts. Les informations ne sont donc pas validées.